After Nelson, 1994, with species from Froese & Pauly, 2001 [“The Fishbase”]

<==o Cyprinodontini ()?
   |-- Cualac tessellatus Miller, 1956 (Checkered pupfish)
   |-- Cyprinodon [?Carrionellus] Olig. R. Eu. L. Tert. R. NA. ()? [N: 36] [FB: 47]
   |--o Floridichthys ()? [2]
   |  |-- F. carpio (Günther, 1866) (Goldspotted killifish)
   |  `-- F. polyommus Hubbs, 1936 (Ocellated killifish)
   |?- Garmanella pulchra Hubbs, 1936 [?Jordanella] (Yucatan flagfish)
   |-- Jordanella floridae Goode & Bean, 1879 (Flagfish)
   `-- Megupsilon aporus Miller & Walters, 1972 (Catarina pupfish)

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