Kari Keinänen - List of publications
2000-present
1995-1999
1990-1994
1984-1989
- Coleman SK, Möykkynen T, Cai C, von Ossowski L, Kuismanen E, Korpi ER and Keinänen K (2006) Isoform specific early trafficking of AMPA receptor flip and flop variants. J Neurosci (in press)
- von Ossowski I, Oksanen E, von Ossowski L, Cai C, Sundberg M, Goldman A and Keinänen K (2006) Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide. FEBS J (in press)
- von Ossowski L, Tossavainen H, von Ossowski I, Cai C, Aitio O, Fredriksson K, Permi P, Annila A and Keinänen K (2006) Peptide binding and NMR analysis of the interaction between SAP97 PDZ2 and GluR-A: Potential involvement of a disulfide bond. Biochemistry 45:5567-5575.
- Cai C, Li H, Rivera C & Keinänen K (2006) Interaction between SAP97 and PSD-95, two Maguk proteins involved in synaptic trafficking of AMPA receptors. J Biol Chem 281:4267-4273.
- Madden DR, Cheng Q, Thiran S, Rajan S, Rigo F, Keinänen K, Reinelt S, Zimmermann H and Jayaraman V (2004) The stereochemistry of glutamate receptor agonist efficacy: engineering a dual-specificity AMPA/kainate receptor. Biochemistry 43: 15838-15844.
- Tichelaar W, Safferling M, Keinänen K, Stark H and Madden DR (2004) The three-dimensional structure of an ionotropic glutamate receptor reveals a dimer-of-dimers assembly. J Mol Biol 344:435-442.
- Pentikäinen OT, Settimo L, Keinänen K and Johnson M (2003) Selective agonist binding of
(S)-2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA) and
2S-(2a,3b,4b)-2-carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid (kainate) receptors:
a molecular modeling study. Biochem Pharmacol 66: 2413-2425
- Pasternack A, Coleman SK, Féthière J, Madden DR, LeCaer J-P, Rossier J, Pasternack M and Keinänen K (2003)
Characterization of the functional role of the N-glycans in the AMPA receptor ligand-binding domain.
J Neurochem (in press)
- Haruyama T, Bongsebandhu-Phubhakdi S, Nakamura I, Mottershead D, Keinänen K, Kobatake E and Aizawa M (2003)
A biosensing system based on extracellular potential recording of ligand-gated ion channel function overexpressed in insect cells. Anal Chem 75: 918-921.
- Coleman SK, Cai C, Mottershead DG, Haapalahti J-P and Keinänen K (2003)
Surface expression of GluR-D AMPA receptor is dependent upon an interaction between its C-terminal domain and a 4.1 protein. J Neurosci 23: 798-806.
- Pasternack A, Coleman SK, Jouppila A, Mottershead DG, Lindfors M, Pasternack M and Keinänen K (2002)
Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels lacking the N-terminal domain. J Biol Chem 277: 49662-49667.
- Jouppila A, Pentikäinen OT, Settimo L, Nyrönen T, Haapalahti JP, Lampinen M, Mottershead DG, Johnson MS and Keinänen K (2002)
Determinants of antagonist binding at the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunit, GluR-D. Role of the conserved arginine 507 and glutamate 727 residues. Eur J Biochem 269: 6261-6270.
- Lampinen M, Settimo L, Pentikäinen OT, Jouppila A, Mottershead D, Johnson MS and Keinänen K (2002)
Discrimination between agonists and antagonists by the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid -selective glutamate receptor.
A mutation analysis of the ligand-binding domain of GluR-D subunit. J Biol Chem 277: 41940-41947.
- Cai C, Coleman SK, Niemi K and Keinänen K (2002)
Selective binding of synapse-associated protein 97 to GluR-A
alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor subunit
is determined by a novel sequence motif. J Biol Chem 277: 31484-31490
- Safferling M, Tichelaar W, Kümmerle G, Jouppila A, Kuusinen A, Keinänen K and Madden DR (2001)
First images of a glutamate receptor ion channel: oligomeric state and molecular dimensions of GluRB homomers. Biochemistry 40: 13948-13953.
- Hirata Y, Laukkanen M-L, Keinänen K, Shigematsu H, Aizawa M and Mizutani F (2001)
Microscopic characterization of Langmuir-Blodgett films incorporating biosynthetically lipid-tagged antibody. Sensors and Actuators B 76: 181-186.
- Abele R, Keinänen K and Madden DR (2000) Agonist-induced isomerization in a glutamate receptor ligand-binding domain. A kinetic and mutagenetic analysis. J Biol Chem 275:21355-21363.
- Madden DR, Abele R, Andersson A and Keinänen K (2000) Large-scale expression and thermodynamic characterization of a glutamate receptor agonist-binding domain. Eur J Biochem 267:4281-4289.
- Féthière J, Anderson A, Keinänen K and Madden DR (2000)
Crystallization of an AMPA receptor binding domain without agonist: importance of carbohydrate content and crystal freezing. Acta Cryst D 56: 1625-1629.
- Jespersen LK, Kuusinen A, Orellana A, Keinänen K and Engberg J (2000) Use of proteoliposomes to generate phage antibodies against native AMPA receptor. Eur J Biochem 267: 1382-1389.
- Flicker S, Laffer S, Steinberger P, Alhani B, Zhu Y, Laukkanen M-L, Keinänen K, Kraft D and Valenta R (2000)
Engineering, purification and applications of His-tagged recombinant antibody fragments with specificity for the major birch pollen allergen, Bet v1. Biol Chem 381: 39-47.
- Kobatake E, Yun K, Yanagida Y, Haruyama T, Keinänen K and Aizawa M (2000)
Immunoassay systems based on immunoliposomes consisting of genetically engineered single-chain antibody. Sensors and Actuators B 65: 42-45.
- Abele R, Svergun D, Keinänen K, Koch, MHJ and Madden D (1999) A molecular envelope of the ligand-binding domain of a glutamate receptor in the presence and absence of agonist. Biochemistry 38: 10949-10957.
- Kuusinen A, Abele R, Madden DR and Keinänen K (1999) Oligomerization and ligand-binding properties of the ectodomain of the AMPA receptor subunit GluRD. J Biol Chem 274: 28937-28943.
- Orellana A, Mottershead D, van der Linden I, Keinänen K and Oker-Blom C (1999) Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes. J Biotechnol 75:209-19.
- Lampinen M, Pentikäinen O, Johnson M and Keinänen K (1998) AMPA receptors and bacterial periplasmic amino acid-binding proteins share the ionic mechanism of ligand recognition. EMBO J 17: 4704-4711.
- Abele R, Lampinen M, Keinänen K and Madden D (1998) Disulfide bonding and cysteine accessibility in the AMPA receptor subunit GluRD: implications for redox modulation of glutamate receptors. J Biol Chem 273: 25132-25138.
- Keinänen K, Jouppila A and Kuusinen A (1998) Characterization of the kainate-binding domain of the glutamate receptor GluR-6 subunit. Biochem. J. 330: 1461-1467.
- Yun K, Kobatake E, Haruyama T, Laukkanen M-L, Keinänen K and Aizawa M (1998) Use of a quartz crystal microbalance to monitor immunoliposome-antigen interaction. Analytical Chemistry 70: 260-264.
- Schmidt M, Tuominen N, Johansson T, Weiss SA, Keinänen K and Oker-Blom C (1998) Baculovirus-mediated large-scale expression and purification of a polyhistidine-tagged Rubella virus capsid protein. Protein Exp Purif 12: 323-330.
- Kobatake E, Sasakura H, Haruyama T, Laukkanen M-L, Keinänen K and Aizawa M (1997)
A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. Analytical Chemistry 69: 1295-1298.
- Keinänen K, Arvola M, Kuusinen A and Johnson M (1997) Ligand recognition in glutamate receptors: Insights from mutagenesis of the soluble AMPA-binding domain of GluR-D. Biochem Soc Trans 25: 835-838.
- Szardenings M, Törnroth S, Mutulis F, Muceniece R, Keinänen K, Kuusinen A and Wikberg J (1997) Phage display selection on whole cells yields a peptide specific for melanocortin receptor 1. J Biol Chem 272: 27943-27948.
- Mottershead D, van der Linden I, von Bonsdorff, C-H, Keinänen K and Oker-Blom C (1997) Baculoviral display of the green fluorescent protein and rubella virus envelope glycoproteins. Biochem Biophys Res Commun 238: 717-722.
- Schiöth H, Kuusinen A, Muceniece R, Szardenings M, Keinänen K and Wikberg JES (1996) Expression of functional melanocortin 1 receptors in insect cells. Biochem Biophys Res Commun 221: 807-814.
- Arvola M. and Keinänen K (1996) Characterization of the ligand-binding domains of glutamate receptor (GluR)-B and GluR-D subunits expressed in Escherichia coli as periplasmic proteins. J Biol Chem 271: 15527-15532.
- Orellana A, Laukkanen M-L and Keinänen (1996) Lanthanide chelate-loaded liposomes: a tool for the study of binding and integrity of liposomes. Biochim Biophys Acta 1284: 29-34.
- Sizmann D, Kuusinen H, and Keränen S, Lomasney J, Caron MC, Lefkowitz RJ and Keinänen K (1996) Production of adrenergic receptors in yeast. Receptors and Channels 4:197-203.
- Oker-Blom C, Orellana A and Keinänen K (1996) Highly efficient production of GFP and its derivatives in insect cells for visual in vitro applications. FEBS Lett 389: 238-243.
- Laukkanen M-L, Oker-Blom C and Keinänen K (1996) Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells. Biochem Biophys Res Commun 226: 755-761.
- Kuusinen A, Arvola M and Keinänen K (1995) Molecular dissection of the ligand binding site of an AMPA receptor. EMBO J 14: 6327-6332.
- Kuusinen A, Arvola M, Oker-Blom C and Keinänen K (1995) Purification of recombinant GluR-D glutamate receptor produced in Sf21 insect cells. Eur J Biochem 233: 720-726.
- Laukkanen M-L, Orellana A. and Keinänen K (1995) Use of genetically engineered lipid-tagged antibody to generate functional europium-chelate-loaded liposomes: Application in fluoroimmunoassay. J Immunol Meth 185: 95-102.
- Keinänen K, Köhr G, Seeburg PH, Laukkanen M-L and Oker-Blom C (1994) High-level expression of functional glutamate receptor channels in insect cells. Bio/Technology 12: 802-806
- Keinänen K and Laukkanen M-L (1994) Biosynthetic lipid-tagging of antibodies. FEBS Lett 346: 123-126.
- Laukkanen M-L, Alfthan K and Keinänen K (1994) Functional immunoliposomes harboring a biosynthetically lipid-tagged antibody. Biochemistry 33: 11664-11670.
- Laukkanen M-L, Teeri T and Keinänen K (1993) Lipid-tagged antibodies: bacterial expression and characterization of a lipoprotein-single-chain antibody fusion protein. Protein Eng 6: 449-454.
- Sommer B, Burnashev N, Verdoorn TA, Keinänen K, Sakmann B and Seeburg PH (1992) A glutamate receptor channel with high affinity for domoate and kainate. EMBO J 11: 1651-1656.
- Lomeli H, Wisden W, Köhler M, Keinänen K, Sommer B and Seeburg PH (1992) High-affinity kainate and domoate receptors in rat brain. FEBS Lett 307: 139-143.
- Remes J, Keinänen K, Petäjä-Repo U and Rajaniemi H (1992) Covalent crosslinking of radiolabeled N-formylated hexapeptide to its specific receptor on rat and human neutrophils: Evidence for a ligand induced complex formation. J Receptor Res 12: 507-527.
- Werner P, Voigt M, Keinänen K, Wisden W and Seeburg PH (1991) Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cells. Nature 351: 742-744.
- Wisden W, Herb A, Wieland H, Keinänen K, Lüddens H and Seeburg PH (1991) Cloning, pharmacological characterization and expression pattern of the rat GABAA receptor alpha-4 subunit. FEBS Lett 289: 227-230.
- Lakkakorpi JT, Keinänen KP and Rajaniemi HJ (1991) Two polyclonal antisera to rat luteal LH/CG receptor with different ligand binding inhibition and immuno-histochemical receptor detection capabilities. Acta Endocrinol 125: 305-312.
- Lakkakorpi JT, Keinänen KP and Rajaniemi HJ (1990) Production and characterization of polyclonal antiserum to rat luteinizing hormone/chorionic gonadotropin receptor and its immunochemical application for studying receptor location and down-regulation. Endocrinology 127: 513-522.
- Eva C, Keinänen K, Monyer H, Seeburg P and Sprengel R (1990) Molecular cloning of novel G-protein-coupled receptor that may belong to the neuropeptide receptor family. FEBS Lett 271: 81-84.
- Sommer B, Keinänen K, Verdoorn TA, Wisden W, Burnashev N, Herb A, Köhler M, Takagi T, Sakmann B and Seeburg PH (1990) Flip and flop: A cell-specific functional switch in glutamate-operated channels of the CNS. Science 249: 1580-1585.
- Lüddens H, Pritchett DB, Köhler M, Killisch I, Keinänen K, Monyer H, Sprengel R and Seeburg PH (1990) Cerebellar GABA-A receptor selective for a behavioural alcohol antagonist. Nature 346: 648-651.
- Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B and Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249: 556-560.
- Ymer S, Draguhn A, Wisden W, Werner P, Keinänen K, Schofield P, Sprengel R, Pritchett DB and Seeburg PH (1990) Structural and functional characterization of the gamma-1 subunit of GABA-A/benzodiazepine receptors. EMBO J 9: 3261-3267.
- Rajaniemi HJ, Keinänen KP, Kellokumpu S, Petäjä-Repo U and Metsikkö MK (1989) Molecular structure of the luteinizing hormone receptor. Biol Reprod 40: 1-12.
- Keinänen KP (1988) Effect of deglycosylation on the structure and hormone-binding activity of the lutropin receptor. Biochem J 256: 719-724.
- Keinänen KP and Rajaniemi HJ (1988) Solubilization of luteinizing hormone receptor from human corpora lutea in a stable form and identification of the hormone-binding unit by ligand blotting. J Clin Endocrinol Metab 67: 228-233.
- Keinänen KP, Kellokumpu S and Rajaniemi HJ (1987) Visualization of the rat ovarian lutropin receptor by ligand blotting. Mol Cell Endocrinol 49: 33-38.
- Keinänen KP, Kellokumpu S, Metsikkö MK and Rajaniemi HJ (1987) Purification and partial characterization of rat ovarian lutropin receptor. J Biol Chem 262: 7920-7926.
- Petäjä U, Kellokumpu S, Keinänen K, Metsikkö K and Rajaniemi HJ (1987) Covalent cross-linking of radiolabeled human chorionic gonadotropin to rat ovarian luteinizing hormone receptor with glutaraldehyde. J Receptor Res 7: 809-825.
- Keinänen KP and Rajaniemi HJ (1986) Rat ovarian lutropin receptor is a transmembrane protein. Evidence of an Mr-20000 cytoplasmic domain Biochem J 239: 83-87.
- Rajaniemi HJ, Keinänen KP, Kellokumpu S and Metsikkö MK (1986) Structure and membrane topography of the lutropin receptor. Biochem Soc Trans 15: 55-57.
- Sotaniemi EA, Keinänen K, Lahtela JT, Arranto AJ and Kairaluoma M (1985) Carbohydrate intolerance associated with reduced hepatic glucose phosphorylating and releasing enzyme activitites and peripheral insulin resistance in alcoholics with liver cirrhosis. J Hepatol 1: 277-290.
- Stengård JH, Saarni HU, Stenbäck F, Keinänen K, Kärki NT and Sotaniemi EA (1985) Medroxyprogesterone acetate and phenobarbital induce NADPH producing enzyme activities in rats with a chemical liver injury. Res Commun Chem Path Pharmacol 50: 93-102.
- Sotaniemi EA, Stengård J, Saarni HU, Arranto AJ, Keinänen K, Kerola T and Sutinen S (1984) Hepatic glucose-6-phosphatase activity in noninsulin-dependent diabetics. Acta Med Scand 215: 323-331.
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